A unique protein labeling system based on melittin and the non-covalent binding-induced pyrene excimer.
نویسندگان
چکیده
We report a unique protein labeling system based on melittin and a pyrene derivative (1). The specific region of the C-terminal in melittin efficiently induced the formation of the pyrene eximer, which can be used as a tag to target proteins and for further detection.
منابع مشابه
Effect of vitamin A compounds on the covalent binding of benzo(a)pyrene to nuclear macromolecules.
Effect of vitamin A compounds on the covalent binding of benzo(a)pyrene [B(a)P] to subnuclear components was studied using rat liver nuclei in vitro. The retinol-induced inhibition of the covalent binding of B(a)P depended both on the dose and the time of addition. Retinal and retinyl acetate were both potent inhibitors, but retinoic acid was less effective. Retinol induced marked inhibition of...
متن کاملLight-switching excimer probes for rapid protein monitoring in complex biological fluids.
Quantitative protein bioanalysis in complex biological fluids presents considerable challenges in biological studies and disease diagnosis. The major obstacles are the background signals from both the probe and the biological fluids where the proteins reside. We have molecularly engineered light-switching excimer aptamer probes for rapid and sensitive detection of a biomarker protein, platelet-...
متن کاملA Pyrene Maleimide with a Flexible Linker for Sampling of Longer Inter-Thiol Distances by Excimer Formation
Pyrene-containing compounds are commonly used in a number of fluorescence-based applications because they can form excited-state dimers (excimers) by stacking interaction between excited-state and ground-state monomers. Their usefulness arises from the facts that excimer formation requires close proximity between the pyrenes and that the excimer emission spectrum is very different from that of ...
متن کاملPreparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c
Objective(s): The present work deals with the preparation of nanobioconjugates based on the immobilization of cytochrome c (cyt c) on functionalized multi-wall carbon nanotubes (f-MWCNTs). The effect of the nanosupport and the immobilization procedure on the biochemical and structural characteristics of the immobilized protein was investigated. Methods: </strong...
متن کاملLysine triggered ratiometric conversion of dynamic to static excimer of a pyrene derivative: aggregation-induced emission, nanomolar detection and human breast cancer cell (MCF7) imaging.
A simple pyrene based probe (A3) derived from pyrene-1-carboxaldehyde and 2-amino-1-phenylpropan-1-ol shows unique optical response triggered by the concentration of lysine (Lys). This allows selective nanomolar detection of Lys via a cascade of processes, dynamic to static-excimer conversion in a ratiometric manner followed by aggregation-induced emission (AIE). Imaging of Lys in the human bre...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 46 21 شماره
صفحات -
تاریخ انتشار 2010